鄰近編碼技術

突破性的單外泌體檢測技術，改變了傳統(tǒng)外泌體技術往往局限于總蛋白、總核酸檢測的困境，實現(xiàn)了高精度、高靈敏度的外泌體異質性研究。

鄰近編碼技術（Proximity Barcoding Assay，PBA）文章：Wu et al. (2019) Profiling surface proteins on individual exosomes using a proximity barcoding assay

樣本中單個外泌體的表面蛋白組成通過與之結合的抗體-DNA偶聯(lián)物可被轉化為DNA序列信息。

流程步驟

1、抗體-DNA偶聯(lián)物識別外泌體表面蛋白并結合，PBA技術可以實現(xiàn)同時對單外泌體表面200+蛋白進行識別標記。

2、滾環(huán)DNA序列通過識別單外泌體表面標記物，實現(xiàn)單外泌體分隔并標記。

3、新一代測序技術解析DNA序列中的蛋白與外泌體信息。

4、生物信息學分析單外泌體蛋白組學信息。

創(chuàng)新與優(yōu)勢

1、高通量單外泌體（10萬）分析

對于每個樣本中10萬+的單外泌體蛋白進行同步分析，為您發(fā)現(xiàn)樣本中低豐度外泌體亞群。

2、<5ul樣品量且無需提純

絕大部分僅僅需要微量樣本，無需預先進行外泌體分離純化步驟，進一步節(jié)約時間與實驗成本。

3、單信號分析檢測高靈敏度的數(shù)據(jù)結果，檢測分辨率精確至單外泌體表面分子蛋白，可識別具有更高疾病診斷價值的低豐度外泌體表面蛋白。

4、適用于幾乎所有體液、培養(yǎng)液

例如血漿、尿液、細胞培養(yǎng)液、器官灌洗液均能進行單外泌體檢測，適用樣本類型廣泛，可以實現(xiàn)更多不同種類樣本的單外泌體檢測，大大減少了樣本的各類限制。

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